Endospore staining is a differential stain that aims at detecting, identifying and differentiating an endospore from the vegetative cell (an underdeveloped endospore). Lab 7: Endospore Stain and Bacterial Motility Last updated; Save as PDF Page ID 3446; Contributors and Attributions; A. ENDOSPORE STAIN . Steam from heated water bath supplies heat to drive green stain into endospores; 4 & 5. However, positive results of endospore stain specify that the organism is a type of spore-forming or endospore encompassing organism. Endospore stained slide with + control (Bacillus) on left, negative control on right and unknown in center. 2. THE VIRTUAL EDGE: Lab 4 Bacterial Staining Techniques III Endospore Stain Results: Lab 4 / Endospore Stain / Capsule Stain / Lab 4 Organisms Endospore stains require heat to drive the stain into the cells. For the staining of endospores, a first article was published by Dorner in the year 1922. Identify a bacterium as an endospore-containing Clostridium by its "tennis racquet" appearance. Most failed endospore stains occur because the stain was allowed to completely evaporate during the procedure. State the function of bacterial endospores. Alternate Endospore Stain. Also allows differentiation based on the position of the endospore in the cell Principle: Spores have a durable outer coating that is composed of the protein keratin. ... Gram stain of Serratia marcescens. Bacterial cells stain red; Endospores stain … There has been some concern about using heat in a teaching environment for safety reasons. DISCUSSION . 2. Endospore Stain Results. Endospore staining is a type of special staining method that makes the use of differential staining procedure to highlight the presence or absence of endospores. Stain with malachite green solution for 10 minutes; Rinse thoroughly with water and dry briefly; Counter stain with safranin solution for 15 seconds; Rinse thoroughly with water and blot dry; Look at cells under 40X; Results. Growth On DTC agar. Recognize endospores as the "structures" observed in an endospore stain preparation. Gram staining a sample of the organism from a pure culture revealed that it was a gram negative rod. The Bacillus cereus organism appeared as red rod-shaped chains of cells, measuring 5 um long and 1 um wide. 1, 2 & 3. The organism was able to grow in both aerobic and anaerobic tubes. It is a simpler but less robust procedure. Because of this, an alternative Endospore stain is sometimes used. DISCUSSION. 1. In the endospore stain experiment (Table 2), a slide of Bacillus cereus was observed under 1000X magnification using oil immersion. For a endospore stain to be successful, the temperature of the stain must be near boiling and the stain cannot dry out. RESULTS . This indicates that cells possess the ability to produce spores. Safranin couterstain of endospore staining procedure; 6. 1. RESULTS. Spores were present in the cells, with a short rod-like shape and green color. For best results, follow this procedure … Malachite green staining step of endospore stain procedure. Name two endospore-producing genera of bacteria. Oxidation Fermentation tests. Purpose: To differentiate between organisms that can produce endospores and those that cannot. A. ENDOSPORE STAIN . An alternative endospore stain is sometimes used was able to grow in both aerobic and anaerobic tubes was allowed completely... Teaching environment for safety reasons and those that can not drive the stain can dry! Bacillus cereus organism appeared as red rod-shaped chains of cells, with a short rod-like shape and endospore stain results.. In the year 1922 um long and 1 um wide left, negative control on right and unknown in.... 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